Signs of the Times - DNA Amplification
October 2001
Letters to the Editor: DNA Amplification
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We know that you look to this venue for political and social guidance, but you may not know that, every once in a while, we're sought out for scientific expertise as well:

Dear George,

I am a comedian writing a comedy novel in Western Australia. I went to your Monica Lewinsky website and think that maybe you can help me. So:

If a bedsheet had semen on it, which had dried for twelve hours:

Would DNA testing be possible?

How would it be done?

Could the semen definitely be matched to a specific person?

How long would the process take?

If you can help, great.

Thanks, jude

Judith Bridge
jude@q-net.net.au

We found an expert locally with not much difficulty.

Mr. Loper,

To the best of my knowledge, dried semen can be suspended in buffer and the DNA can be extracted, amplified, and analyzed for various STR polymorphisms.

Although drying is not the best way to preserve any biological specimen, there should not be too much degradation of the DNA in the time period specified.

If the DNA has not undergone much degradation, it should be possible to match the semen to a particular individual. The analysis could be done in a day or two depending on how much one was willing to pay for expedited treatment.

Robert J. Huskey
rjh9u@blue.unix.virginia.edu
Emeritus Professor, Dept. of Biology, UVa

and expect due credit upon publication.


Comments? Questions? Write me at george@loper.org.